The company has launched a high-throughput and highly automated single-cell visualization sorting system based on GRID patent technology (patent number: WO2019197373A1). IsoPick uses micro jet technology to reshape the cell culture medium (or stem cell coating) using interfacial tension, and carves a separate cell chamber GRID on the culture dish. IsoPick can create 256 single cell compartment GRID arrays on a 6cm culture dish and automatically allocate cells to each GRID single cell compartment in nanoliter volume. The single cells in the GRID compartment can be clearly seen through isoPick's optical microscope. Based on GRID technology and optical imaging information, isoPick can ensure that 100% of the sorted cells are single cells.
application area
Single cell sorting
Single cell cloning
Single-cell omics
100% accurate single-cell sorting efficiency
Significantly improve the survival rate of single-cell clones (monoclonal rate)
Greatly simplifying the steps of single-cell omics
Technical advantages
Traditional single-cell sorting methods cannot guarantee that there is only one single cell in the obtained sample, while isoPick uses a sorting technique that combines GRID single-cell chamber separation and optical signal verification to ensure that there is only one single cell in the sorted single-cell sample.
IsoPick can efficiently isolate single cells of hiPSCs for constructing monoclonal cell lines. IsoPick is gentle in handling sensitive single cells, ensuring higher single cell survival rates and achieving better clone growth effects.
IsoPick can directly transfer 1.5-200 µ l single-cell samples to PCR bands or 96 well plates, seamlessly connecting the subsequent single-cell sequencing scWGA process, greatly simplifying the single-cell omics steps.
GRID cell compartments before and after single-cell sorting
Single cell hiPSC colonies coated with 96 well plates of different matrices
WGA results of single cells
Equipment characteristics
-Fully automated processes
-Easy to operate, no damage to cells
-Results can be tracked
-Separation efficiency up to 100%
-Directly transfer to PCR tube or 96 well plate
-Compact structure and small volume
Traditional single-cell separation methods cannot guarantee that the obtained sample contains only one single cell, which may result in multiple cells or cell clusters, leading to errors in downstream experiments. The GRID technology used by isoPick, combined with image information analysis, ensures traceable results and 100% accurate single-cell sorting. Moreover, the isoPick sorting conditions are mild and can significantly improve the survival rate of sorted single cells. At the same time, isoPick can directly transfer single-cell samples to 96 well plates or PCR tubes according to specific volumes, seamlessly connecting downstream single-cell applications and ensuring the integrity of subsequent single-cell omics information.
Application cases
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User unit
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Single cell cloning of human induced pluripotent stem cells (hiPSCs)
The cultivation process of human induced pluripotent stem cells (hiPSCs) to construct monoclonal cell lines is cumbersome, and cells are very sensitive to abnormal handling and manipulation. Traditional single-cell sorting can easily lead to the accumulation of cellular and genotoxic stress, resulting in poor differentiation and loss of pluripotency.
The use of isoPick can gently and automatically separate human induced pluripotent stem cells (hiPSCs) into single cells, enabling efficient cultivation of hiPSC monoclonal cell lines, significantly improving cell separation and cloning efficiency.
