Single cell visualization sorting and culture system - isoCell

Single cell visualization sorting and culture system - isoCell

A high-throughput, highly automated single-cell visual sorting and cultivation system based on GRID patented technology has been launched. IsoCell uses microjet technology to reshape the cell culture medium (or stem cell coating) using interfacial tension, carve out individual cell chamber GRIDs on the culture dish (256 single cell chamber GRID arrays can be created on a 6cm culture dish), and automatically distribute cells in nanoliter volume to each GRID single cell chamber. IsoCell can avoid boundary effects, and its built-in optical microscope can clearly see single cells in the GRID chamber, ensuring that the cells sorted by isoCell are 100% single cells. IsoCell can cultivate single cells into monoclonal cell lines in GRID, and during the cultivation process, the medium can be changed according to customer needs. The entire process is visually monitored to ensure that each monoclonal cell line comes from a selected individual cell.
The isoCell single-cell visualization sorting and cultivation system can achieve high-throughput, automated, and high survival rate monoclonal cell line construction, microbial sorting and cultivation, single-cell sorting, single-cell omics, and other functions.
application area
Construction of Monoclonal Cell Lines
Single cell sorting
Single-cell omics
Efficient, mild, and highly automated construction of monoclonal cell lines. 100% accurate single-cell sorting greatly simplifies the steps of single-cell omics
Technical advantages
Traditional techniques for constructing monoclonal cell lines are limited by the high sensitivity and low success rate of single cells, and cannot guarantee that each monoclonal cell line comes from a single cell. IsoCell adopts GRID single-cell microcavity technology, which can highly automate and gently cultivate monoclonal cell lines, ensuring a success rate of up to 60% -70% in cultivation. And the entire operation process is visually validated to ensure that each monoclonal cell line comes from a single cell.
Traditional single-cell sorting methods cannot guarantee that there is only one single cell in the obtained sample, while isoCell adopts a sorting technique combining GRID single-cell chamber separation and optical signal verification, which can ensure that there is only one single cell in the sorted single-cell sample.
IsoCell can directly transfer single-cell samples ranging from 1.5 µ l to 200 µ l into PCR tubes or 96 well plates, seamlessly connecting the subsequent single-cell sequencing scWGA process, greatly simplifying the single-cell omics steps.
8-day cultivation of monoclonal cell lines on GRID
GRID cell compartments before and after single-cell sorting
WGA results of single cells
Equipment characteristics
-Fully automated processes
-Mild operating conditions, no damage to single cells
-Visual tracking of the entire training and analysis process
-Single cell separation efficiency up to 100%
-High survival rate of monoclonal cell line construction
-Directly transfer to PCR tube or 96 well plate
-Compact structure and small volume
Traditional single-cell separation methods cannot guarantee that 100% of the obtained sample contains only one single cell, which may result in multiple cells or cell clusters, leading to errors in downstream experiments. Moreover, traditional techniques for constructing monoclonal cell lines are limited by the high sensitivity and low success rate of single cells, and cannot guarantee that each monoclonal cell line comes from a single cell. The GRID single-cell chamber technology adopted by isoCell ensures 100% accurate single-cell sorting and highly automated direct cultivation of monoclonal cell lines in GRID, with a survival rate of over 60%. Through full process visualization monitoring, it ensures that each monoclonal cell line comes from a selected individual cell. IsoCell sorting and mild cultivation conditions can significantly improve the survival rate of single cell clones. At the same time, isoCell can directly transfer single-cell samples to 96 well plates or PCR tubes according to specific volumes, seamlessly connecting downstream single-cell applications and ensuring the integrity of subsequent single-cell omics information.